Hi,
I have another question following Macrophage staining. I am setting up a Laser Capture Microdissection protocol to dissect macrophages from atherosclerosis lesions (non fixed frozen sections) and extract RNA for gene expression purpose. I am looking at a quick staining (less than 30min otherwise RNA is degradated) for macrophages in that context? Any suggestions? Thanks a lot, Delphine Delphine Eberlé PhD UCSF Department of Vascular Surgery eberledelph...@hotmail.com > From: jkier...@uwo.ca > To: sharon.will...@bms.com > Date: Wed, 27 Jan 2010 00:22:53 -0500 > Subject: Re: [Histonet] Histology Special Stains for Macrophages > CC: histonet@lists.utsouthwestern.edu > > None of the methods mentioned in the enquiry are stains for macrophages. > Research workers who never took Histology 101 often stain cells of the > monocyte/macrophage lineage immunohistochemically (IHC), using very expensive > primary antibodies and fairly expensive kits to amplify and detect the > binding sites. IHC is necessary if you must find every macrophage, including > a tissue's recent monocyte immigrants that haven't yet done any work. > Macrophages that have been busily eating blood are full of brown granules > that don't need any staining. > > Ordinary people recognize macrophages by their appearance in sections stained > with a well done H&E or with one of the many Romanowsky-Giemsa blood stains. > With the latter it's important to get the pH right - much lower for sections > of formaldehyde-fixed objects (pH4 to 5) than for methanol-fixed films or > smears (traditionally 6.8). It's all very well explained in RD Lillie & GM > Fullmer's "Histopathologic Technic..." (4th ed 1976, pp.193-197; the 3rd > edition, 1965, is OK too) and also in more recent textbooks including those > by Bancroft & Gamble, Geoffrey Brown, Freida Carson and, of course > Yours truly. > > John Kiernan > Anatomy, UWO > London, Canada > http://publish.uwo.ca/~jkiernan/bookfind.htm > = = = > ----- Original Message ----- > From: "Willman, Sharon" <sharon.will...@bms.com> > Date: Tuesday, January 26, 2010 12:12 > Subject: [Histonet] Histology Special Stains for Macrophages > To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> > > > Hi, > > We are needing to do a special stain for macrophages. What > > is the most common stain for that? Does anyone do a Sudan > > Black, Alcian Blue or Van Gieson for macrophages? Any > > information would be appreciated. > > Thanks in advance. > > Sharon > > > > > > ________________________________ > > This message (including any attachments) may contain > > confidential, proprietary, privileged and/or private > > information. The information is intended to be for the use of > > the individual or entity designated above. If you are not the > > intended recipient of this message, please notify the sender > > immediately, and delete the message and any attachments. Any > > disclosure, reproduction, distribution or other use of this > > message or any attachments by an individual or entity other than > > the intended recipient is prohibited. > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _________________________________________________________________ Téléchargez Internet Explorer 8 et surfez sans laisser de trace ! http://clk.atdmt.com/FRM/go/182932252/direct/01/_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet