Hello,
We are working on an oncology project that requires the administration of fluorescent dyes in tumor bearing mice. The dyes are allowed to circulate and then tumors are collected, homogenized and centrifuged for fluorescent reading. Specifically, we are looking at the dyes in the interstitial fluid and we cannot have any contamination from blood. We are getting a lot of background fluorescence and we think it may be coming from blood in the tissue. We want to perfuse the animal before tumor collection but we think that if we perfusing with saline will affect the interstitial fluid as well. Has anyone tried to perfuse with something that has high molecular weight? Any advice, tips on this? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet