This sounds a lot like what I did for my master's project. What I believe I
did to correct for the autofluorescence from RBCs was to have a group of
control tumor-bearing mice perfused with saline only, take readings as you
would with the dye samples, and in the analysis subtract out the saline
readings as background. In theory you should have a similar amount of blood
in your saline samples as in your dye samples, if your groups are large
enough to account for mouse-to-mouse (or tumor-to-tumor) variability (which
I found tended to be rather large...).
Regards,
Merced
Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
--On Monday, February 22, 2010 10:40 AM -0800 Wisam Barkho
<hermeneu...@gmail.com> wrote:
Hello,
We are working on an oncology project that requires the administration of
fluorescent dyes in tumor bearing mice. The dyes are allowed to circulate
and then tumors are collected, homogenized and centrifuged for fluorescent
reading. Specifically, we are looking at the dyes in the interstitial
fluid and we cannot have any contamination from blood. We are getting a
lot of background fluorescence and we think it may be coming from blood
in the tissue. We want to perfuse the animal before tumor collection but
we think that if we perfusing with saline will affect the interstitial
fluid as well. Has anyone tried to perfuse with something that has high
molecular weight? Any advice, tips on this?
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
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