We have a difficulty cutting metatarsal bone . It seems that our sections are so dried up. I was thinking that our dehydration have something to do with this which we have placed it in a wrong processing procedure for our large bone. The tissue is 4 mm thick and 1-2 cm in length and width and was dehydrated in 70% - 4 hrs, 80%-4 hrs,95% -4 hrs and 2 changes of 100% 3 hrs each, paraffin is 4 hrs each 2 changes. The tissue was decalcified in 14% EDTA. When we start cutting them it is so brittle and we could not even create a section. I have surfaced decal it and also place in a softener Mollifex some of it work but some does not work. Please help us remedy this tissue. Thank you.
Reuel Cornelia, BS MT, AMT Cellular Pathology Texas Scottish Rite Hospital for Children 2222 Welborn Street Dallas, TX 75219 Tel: 214-559-7766 fax: 214-559-7768 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
