Hello, I'm glad that you already solved the problem your way.
I didn't read your first post, but we had exactly the same problem (and we're also using cover plates). This artifact is very likely caused by tiny air bubbles which are trapped under the cover plate. The crucial step is when you drip a little buffer onto the plate in order to get your slide in proper position. You get much lesser air bubbles if a) no detergent is used and b) the PBS or TBS is at room temperature and not cold (which isn't good anyway). We don't use detergents anymore (on coverplates). Alexandra Meinl ************************************************ Dr. Alexandra Meinl Ludwig Boltzmann Institute for Experimental and Clinical Traumatology Austrian Cluster for Tissue Regeneration Histology Donaueschingenstrasse 13 1200 Vienna - Austria Contact @ Bernhard Gottlieb University School of Dentistry, Waehringerstr. 25a, A-1090 Vienna tel: +43 1 4277 67026 fax: +43 1 4277 67019 email: alexandra.me...@trauma.lbg.ac.at _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
