Stephen, we doubled the routine protocol for braintissue from 13 to 24 hour. We stayed at one hour for the formalin steps, because the tissue is fixed for several days before processing. This protocol is also used for prostate big cassettes. Gudrun
-----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Stephen Kum Jew Gesendet: Mittwoch, 03. März 2010 21:43 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: paraffin processing times for brain Hi all I was wondering if anyone can send me please the optimal paraffin processing times for brain autopsy tissue. Currently the blocks have cut and stained well but 4-5mm thick tissue has expanded (bulged out) following cutting as if they had absorbed water. Solutions been all changed. thanks Stephen |Stephen Kum Jew |Senior Technical Officer |Discipline of Pathology |School of Medical Sciences |Blackburn Building D06 |University of Sydney NSW 2006 |Australia |Ph: + 61 2 9036 9027 |Fax:+61 2 9351 3429 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet