Running tap water for 10 minutes should do the trick. Joe Saby
________________________________ From: Cynthia Pyse <cp...@x-celllab.com> To: cscam...@uci.edu; HistoNet <histonet@lists.utsouthwestern.edu> Sent: Tue, March 16, 2010 10:52:20 AM Subject: RE: [Histonet] Removing Yellow color from slides refixed in Bouin's solution I just place the slides into 80% ETOH for 2 minutes. Cindy -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of cscam...@uci.edu Sent: Monday, March 15, 2010 7:42 PM To: HistoNet Subject: [Histonet] Removing Yellow color from slides refixed in Bouin's solution Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichrome procedure? Thanks! -Colin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
