Li,
Mouse cardiac output seems to be about 17 ml/min (e.g.
www.transonic.com/mice1.shtml), you probably want to try for that to
keep pressures close to physiological.
A syringe pump is pretty inexpensive and probably all you need.
Mike
----- Original Message -----
From: Li Zhang <dancingw...@yahoo.com>
Date: Wednesday, March 17, 2010 14:59
Subject: [Histonet] question about mouse perfusion
To: histonet@lists.utsouthwestern.edu
> > My question is: can anyone give me a rough idea of how fast I
> > should inject ( like ml/min). I think I've tried like 30 ml in 3
> > min, and I suspect that it's too fast because I do observe
> > tissue swelling sometimes.
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