Roger The tissue may be sightly harder and a little more difficult therefore to section but apart from that don't see any real problems. You may not notice any difference. Barry
________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger [rchar...@state.pa.us] Sent: Friday, March 19, 2010 7:57 AM To: Histonet (histonet@lists.utsouthwestern.edu) Subject: [Histonet] Processor malfunction Happy Friday to all, Our processor shut off last evening in 80% alcohol due to a misconnection of the next reagent. We restarted this morning when we arrived but the tissues were sitting in 80% for 9 hours. My question is what affect will this have on the tissues which are a mixture of medulla and lymph node sections? Thanks roger Roger Charles Microbiologist II PA Veterinary Laboratory 717-787-8808 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet