I use VWR VistaVision coverslips (for fluorescent imaging) and had noticed
some dust/debris on them that would autofluoresce and give false positives
with some of my stains. The problem disappeared when I got into the habit
wiping all my coverslips with EtOH before mounting...this may be cumbersome
if you're doing a lot of slides, however. But it has worked really well.
Regards,
Merced
--On Wednesday, March 24, 2010 4:24 PM -0400 Helen Fedor <hfe...@jhmi.edu>
wrote:
Hello, Our lab does IHC staining in house and have had zero problems with
our Fisher brand cover slips until recently. We've tried both Fisher and
Corning cover slips and what appears to be dust or imperfections in the
glass are found on both brands. This is creating false positives for us,
because when the positive staining is low it is difficult to impossible
to tell the imperfections from the staining itself.
Any advice will be welcome.
--
Helen
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Merced M Leiker
Research Technician III
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
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