We inflate our lung tissue with a thin OCT compound (50% OCT/ 50% 0.5M sucrose mixture) using a needle and syringe and then embed the lung in 100% OCT and snap freeze. It helps for interpretation to have open alveoli and also gives the tissue some stability internally for frozen sections.
Megan K. Dishop MD Department of Pathology, B120 The Children's Hospital 13123 E. 16th Avenue Aurora, CO 80045 Ph: 720-777-4337 Fax: 720-777-7119 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Molinari, Betsy Sent: Wednesday, April 07, 2010 8:46 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] frozen lung sections Hi all, I have received some lung samples on OCT. These lungs have not been in PBS or any fixative. They went directly from dissection into OCT and frozen. I cannot get a section. The temp of the cryostat is -20. I have done some reading and it seems the best results come from immersion in PBS, then 4% paraformaldehyde then into sucrose. Can anyone clarify the procedure or offer advice? I am unfamiliar with this technique since most of my frozen sectioning is heart tissue and it does not present the same problem as the lung. Thanks, Betsy Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave MC1-283 Houston,TX 77030-2607 832-355-6524 832-355-6812 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This e-mail is confidential, may be legally privileged, and for the intended recipient only. Access, disclosure, copying, forwarding and distribution by any means is strictly prohibited. If received in error, do not read but delete and e-mail confirmation to the sender. ================================================================================ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet