please add me to the growing list as well. thanks, IS. ----------------------------------------------------- Irini Skaliora, PhD Research Assistant Professor Developmental Biology Division Biomedical Research Foundation of the Academy of Athens (BRFAA) Soranou Efessiou 4 Athens 11527 tel. +30-210-6597203 (office) tel. +30-210-6597482 (lab) fax. +30-210-6597545 email: iskali...@bioacademy.gr
> Me three, please. :-) > > I just discovered we have this same antibody in our lab but my boss was > unsuccessful with it (he had tried 95oC for 45 min, pH 6.0). > > Thanks! > > Merced > > --On Tuesday, April 13, 2010 2:24 PM -0500 shive...@umn.edu wrote: > >> Me, too, please. >> >> Jan Shivers >> UMN Vet Diag Lab >> >> On Apr 12 2010, Liz Wilson wrote: >> >>> Hi Patsy, >>> I would love to try the method! Thanks -Liz >>> >>> Liz Wilson >>> Bethyl Labs >>> >>> >>> ________________________________________________________________________ >>> ____ Message: 7 >>> Date: Sat, 10 Apr 2010 10:33:02 -0600 >>> From: "Patsy Ruegg" <pru...@ihctech.net> >>> Subject: RE: SPAM-LOW: [Histonet] Re: Cd31 in pigs >>> To: "'Liz Wilson'" <lwil...@bethyl.com>, >>> <histonet@lists.utsouthwestern.edu> >>> Message-ID: <59adba33d53b44778565f88cbd7fa...@prueggihctechlt> >>> Content-Type: text/plain; charset="us-ascii" >>> >>> A few years ago I spent a lot of effort on using Serotec's mouse >>> anti-porcine CD31 for ffpe porcine tissue and I did get some positive >>> results with unusual pretreatment methods, as I recall I had to use a >>> waterbath for hier set at 70dc for 3 hours in hph buffer. I will look >>> up >>> the method at work on Monday and send it to you if you contact me again >>> to remind me. >>> >>> Patsy >>> >>> Patsy Ruegg, HT(ASCP)QIHC >>> IHCtech >>> 12635 Montview Blvd. Ste.215 >>> Aurora, CO 80045 >>> 720-859-4060 >>> fax 720-859-4110 >>> www.ihctech.net >>> www.ihcrg.org >>> >>> >>> _______________________________________________ >>> Histonet mailing list >>> Histonet@lists.utsouthwestern.edu >>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >>> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > > Merced M Leiker > Research Technician III > Cardiovascular Medicine > 348 Biomedical Research Building > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 USA > lei...@buffalo.edu > 716-829-6118 (Ph) > 716-829-2665 (Fx) > > No trees were harmed in the sending of this email. > However, many electrons were severely inconvenienced. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet