Life is uncertain, but........ We may have an opening in the future. You may send resumes directly to this email address and I will forward them on to the appropriate people.
Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Monday, April 26, 2010 10:02 AM To: histonet@lists.utsouthwestern.edu Subject: [BULK] Histonet Digest, Vol 77, Issue 32 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: [IHCRG] ER clone 1D5 or SP1 ? (Richard Cartun) 2. Region 1 conference (Amos Brooks) 3. Re: [IHCRG] ER clone 1D5 or SP1 ? (ancillaryp...@mac.com) 4. Mysterious artifact on GI biopsies H&E stain (Urim, Lyudmila) 5. paraformaldayhde (Perry, Margaret) 6. RE: Mysterious artifact on GI biopsies H&E stain (Weems, Joyce) 7. anti 8 hydroxyguanosine (Fabrice gankam) 8. RE: Mysterious artifact on GI biopsies H&E stain (Mike Pence) 9. Any Histology openings in San Diego area? (Jill Cox) 10. (no subject) (Urim, Lyudmila) 11. Re: Two antibodies from the same host with tyramide (Johnson, Teri) 12. RE: (no subject) (Kaye Ryan) 13. RE: (no subject) (Nails, Felton) 14. RE: Best books for the HTL? (Morken, Tim) 15. RE: (no subject) (Jesus Ellin) 16. RE: [IHCRG] ER clone 1D5 or SP1 ? (Van Eyck, Deb) 17. RE: [IHCRG] ER clone 1D5 or SP1 ? (Patsy Ruegg) ---------------------------------------------------------------------- Message: 1 Date: Sun, 25 Apr 2010 15:04:28 -0400 From: "Richard Cartun" <rcar...@harthosp.org> Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? To: "'ih...@googlegroups.com'" <ih...@googlegroups.com>, "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu>, <jtay...@meriter.com> Message-ID: <4bd459fb.7400.007...@harthosp.org> Content-Type: text/plain; charset=US-ASCII I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax >>> "Taylor, Jean" <jtay...@meriter.com> 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en ------------------------------ Message: 2 Date: Sun, 25 Apr 2010 19:14:49 -0400 From: Amos Brooks <amosbro...@gmail.com> Subject: [Histonet] Region 1 conference To: histonet@lists.utsouthwestern.edu Message-ID: <y2z582736991004251614m979ddea5yea99650b77ced...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hi, I would like to ask if anyone has any photos of the Region 1 conference to consider emailing them to me. I was going to post some on the Region 1 Conference web page and possibly put some in the Paraffin Press. If you are interested, please drop me a line. Thanks, Amos amosbro...@gmail.com ------------------------------ Message: 3 Date: Sun, 25 Apr 2010 20:36:18 -0400 From: ancillaryp...@mac.com Subject: [Histonet] Re: [IHCRG] ER clone 1D5 or SP1 ? To: "ihcrg Group (E-mail)" <ih...@googlegroups.com>, histonet netserver <histonet@lists.utsouthwestern.edu> Message-ID: <0d48ea46-525d-45a6-bfcd-24662d59f...@mac.com> Content-Type: text/plain; charset=us-ascii; format=flowed; delsp=yes When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: > I have looked at several clones over the years and I prefer clone > 6F11. > > Richard > > Richard W. Cartun, Ph.D. > Director, Histology & Immunopathology > Director, Biospecimen Collection Programs > Assistant Director, Anatomic Pathology > Hartford Hospital > 80 Seymour Street > Hartford, CT 06102 > (860) 545-1596 Office > (860) 545-2204 Fax > > >>>> "Taylor, Jean" <jtay...@meriter.com> 4/23/2010 11:17 AM >>> > > I'm wondering which clone of ER most labs are using? > > Thanks, > Jean Taylor, HT(ASCP)QIHC > IHC Tech > Meriter Health Services > Madison, WI > > > -- > Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en > ------------------------------ Message: 4 Date: Mon, 26 Apr 2010 07:23:57 -0400 From: "Urim, Lyudmila" <lu...@challiance.org> Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain To: <histonet@lists.utsouthwestern.edu> Message-ID: <6774152b580a6e44b38487b24a331e7c13160...@sasquatch.cphc.local> Content-Type: text/plain; charset="us-ascii" > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. > Also on the affected GI biopsies there is often only 1 or 2 spots of > the biopsy that are affected while the rest of the biopsy looks fine. > The artifact is being reproduced on each cut; also the artifact > doesn't disappear after the affected biopsies get reprocessed. > The artifact can be described as a foggy, smudgy stain, with deformed > nucleus and not enough cell details. > All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy ------------------------------ Message: 5 Date: Mon, 26 Apr 2010 07:43:53 -0500 From: "Perry, Margaret" <margaret.pe...@sdstate.edu> Subject: [Histonet] paraformaldayhde To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fca5ef47f9bc694cbb4c58fea04219634ccfb69...@sdsu-mbx.jacks.local> Content-Type: text/plain; charset="us-ascii" In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? ------------------------------ Message: 6 Date: Mon, 26 Apr 2010 09:48:08 -0400 From: "Weems, Joyce" <jwe...@sjha.org> Subject: [Histonet] RE: Mysterious artifact on GI biopsies H&E stain To: "Urim, Lyudmila" <lu...@challiance.org>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <92ad9b20a6c38c4587a9febe3a30e164015dccb...@chexcms10.one.ads.che.org> Content-Type: text/plain; charset="us-ascii" I would check to see what medication the patient was on or if something was done during the procedure to affect the biopsies.. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 07:24 To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. > Also on the affected GI biopsies there is often only 1 or 2 spots of > the biopsy that are affected while the rest of the biopsy looks fine. > The artifact is being reproduced on each cut; also the artifact > doesn't disappear after the affected biopsies get reprocessed. > The artifact can be described as a foggy, smudgy stain, with deformed > nucleus and not enough cell details. > All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This email, including any attachments is the property of Catholic Health East and is intended for the sole use of the intended recipient(s). It may contain information that is privileged and confidential. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please reply to the sender that you have received the message in error, then delete this message. ------------------------------ Message: 7 Date: Mon, 26 Apr 2010 09:00:32 -0500 From: Fabrice gankam <gan...@googlemail.com> Subject: [Histonet] anti 8 hydroxyguanosine Cc: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <u2k86c37d601004260700ufa940a43t440c8d062826...@mail.gmail.com> Content-Type: text/plain; charset=ISO-8859-1 Hey guys was wondering if any of you used the anti 8 hydroxyguanosine to detect free radical induced DNA and RNa damage. which antibody is the best. we tried the one from abdserotec and it is a disaster. the background is just horrible. any idea ? 2010/4/26 Perry, Margaret <margaret.pe...@sdstate.edu> > In the Brown and Hopps gram stain can I substitute 37 g in 100 ml of > powdered paraformaldyhyde for the 37% formalin or do I need to buffer it? > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 8 Date: Mon, 26 Apr 2010 09:04:43 -0500 From: "Mike Pence" <mpe...@grhs.net> Subject: RE: [Histonet] Mysterious artifact on GI biopsies H&E stain To: "Urim, Lyudmila" <lu...@challiance.org>, <histonet@lists.utsouthwestern.edu> Message-ID: <661949901a768e4f9cc16d8af8f2838c017a3...@is-e2k3.grhs.net> Content-Type: text/plain; charset="us-ascii" It could be a "hot biopsy"? -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 6:24 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Mysterious artifact on GI biopsies H&E stain > Hi, > > We have been getting a similar staining artifact on some GI biopsies > randomly while the rest GI biopsies look fine. Also on the affected > GI biopsies there is often only 1 or 2 spots of the biopsy that are > affected while the rest of the biopsy looks fine. The artifact is > being reproduced on each cut; also the artifact doesn't disappear > after the affected biopsies get reprocessed. The artifact can be > described as a foggy, smudgy stain, with deformed nucleus and not > enough cell details. All our specimens are fixed in 10%NBF. > > Please let me know if you have any ideas. > Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 9 Date: Mon, 26 Apr 2010 07:31:50 -0700 (PDT) From: Jill Cox <jco...@yahoo.com> Subject: [Histonet] Any Histology openings in San Diego area? To: "histo...@lists. Utsouthwestern. Edu" <histonet@lists.utsouthwestern.edu> Message-ID: <519989.77668...@web56803.mail.re3.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi Netters, I have a friend looking to relocate to San Diego area and is looking for a Histology position, she is certified HT. Any help would be greatly appreciated, thanks in advance!! Jill Jill Cox HT (ASCP) Arizona Dermatology 4232 E Cactus Rd Phoenix AZ 85032 ------------------------------ Message: 10 Date: Mon, 26 Apr 2010 11:10:08 -0400 From: "Urim, Lyudmila" <lu...@challiance.org> Subject: [Histonet] (no subject) To: <Histonet@lists.utsouthwestern.edu> Message-ID: <6774152b580a6e44b38487b24a331e7c13160...@sasquatch.cphc.local> Content-Type: text/plain; charset="us-ascii" Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy ------------------------------ Message: 11 Date: Mon, 26 Apr 2010 10:16:26 -0500 From: "Johnson, Teri" <t...@stowers.org> Subject: [Histonet] Re: Two antibodies from the same host with tyramide To: "'anonwu...@gmail.com'" <anonwu...@gmail.com> Cc: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Message-ID: <bd62cbac4395b94096109020651be2ec1301f62...@exchmb-02.stowers-institute. org> Content-Type: text/plain; charset="us-ascii" Adam, Thanks for the detailed protocol. I can't explain it either but I'll throw some ideas out there and maybe it'll stimulate discussion. Hopefully Chris vanderLoos will chime in. Have you tried doing the labeling in reverse, using the VE-Cadherin first and then blocking with the goat IgG, and then doing the other antibody/tyramide protocol? I'm not sure what effect, if any, the hydrogen peroxide might have on the anti-goat fluorescent 649 label, so you could certainly do that block prior to doing the first primary antibody incubation. Also, you may have to double the concentration of the Cadherin to get it to label at the same intensity as you do in single staining. But still I might have expected to see a signal. Do the two antibodies co-express? Could there be something with the covalent binding of the HRP-tyramide complex that shelters the antigenic sites for the second antibody? That's why I wondered what might happen if you reversed your protocol. Let us know if you get it figured out and what fixed it for you. Good luck, Teri Johnson, HT(ASCP)QIHC Managing Director, Histology Facility Stowers Institute for Medical Research Kansas City, MO ------------------------------ Message: 12 Date: Mon, 26 Apr 2010 11:25:50 -0400 From: "Kaye Ryan" <kr...@nfderm.com> Subject: RE: [Histonet] (no subject) To: "Urim, Lyudmila" <lu...@challiance.org>, <Histonet@lists.utsouthwestern.edu> Message-ID: <dcb07c3ef84d92439e5a453d8d5b5eb004a...@exchserver.nfda.com> Content-Type: text/plain; charset="us-ascii" I highly recommend the Milestone microwave tissue processors. I have used their microwave tissues processors in two different locations and have found them to be very reliable, user friendly, reproducible and cost effective. They also have wonderful tech support. Kaye Ryan Histology Supervisor North Florida Dermatology -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 11:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Mon, 26 Apr 2010 10:49:08 -0500 From: "Nails, Felton" <flna...@texaschildrens.org> Subject: RE: [Histonet] (no subject) To: "'Urim, Lyudmila'" <lu...@challiance.org>, "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Message-ID: <c1fe5960057c084ca389ce97779062904ebd2...@tcdmsg01.ad.texaschildrenshosp ital.org> Content-Type: text/plain; charset=us-ascii It really depends on your volume to be processed and what you are planning to process. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 10:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------------------------------------ CONFIDENTIALITY NOTICE: The information in this e-mail may be confidential and/or privileged. If you are not the intended recipient or an authorized representative of the intended recipient, you are hereby notified that any review, dissemination, or copying of this e-mail and its attachments, if any, or the information contained herein is prohibited. If you have received this e-mail in error, please immediately notify the sender by return e-mail and delete this e-mail from your computer system. Thank you. ============================================================ ------------------------------ Message: 14 Date: Mon, 26 Apr 2010 08:54:23 -0700 From: "Morken, Tim" <timothy.mor...@ucsfmedctr.org> Subject: RE: [Histonet] Best books for the HTL? To: "Paula Sicurello" <pat...@gmail.com>, HistoNet <histonet@lists.utsouthwestern.edu> Message-ID: <1aaf670737f193429070841c6b2add4c01818ac...@exmbmcb15.ucsfmedicalcenter. org> Content-Type: text/plain; charset=us-ascii Paula, Carson's book is probably them most relevant to basic histology these days (Sheehans is still very good for detail on technique and stains but has a lot of old material that you will rarely, if ever, see anymore). Bancroft's book is also excellent, has more detail on given stains and has more on management (QC, etc). For the HTL I also recommend the ASCP book "Quality Management in Anatomic Pathology" by Nakhleh and Fitzgibbons. Plus the usual NSH study guides. Tim Morken Supervisor, Histology / IPOX UCSF Medical Center San Francisco, CA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Paula Sicurello Sent: Sunday, April 25, 2010 7:18 AM To: HistoNet Subject: [Histonet] Best books for the HTL? Hello Listers, I am going to study for the HTL exam after being in histology for over 20 years. It seems like more and more labs want the certification and less and less schools are offering the training that's required for the HT/HTL. Anyway, which books are the best to use for the HTL exam? ASCP mentions Bancroft an Gamble "Theory and Practice of Histological Technique" and Garcia "Clinical Laboratory Management". What do you recently certified HTLs think about those suggestions? I'm not getting down on the older certified HTLs but want the opinion of folks who went through the grinder recently. Thanks, -- Paula Sicurello 6 of 6 Duke University EM Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Mon, 26 Apr 2010 09:02:43 -0700 From: "Jesus Ellin" <jel...@yumaregional.org> Subject: RE: [Histonet] (no subject) To: "Urim, Lyudmila" <lu...@challiance.org>, <Histonet@lists.utsouthwestern.edu> Message-ID: <29be166a2cf48d459853f8ec57cd37e8021c6...@exchangecluster.yumaregional.l ocal> Content-Type: text/plain; charset="us-ascii" I would look at this very carefully, especially in light of the new regulation that were just released by the CAP on ER and PR. But that is up to each individual lab Jesus A Ellin HT/PA ASCP Department of Pathology/Histology Yuma Regional Medical Center 2400 South Ave A Yuma, AZ 85364 - 7170 ( Office: (928) 336-1743 ( Fax: (928) 336-7319 * Email: jel...@yumaregional.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila Sent: Monday, April 26, 2010 8:10 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] (no subject) Hi, I am looking into purchasing a microwave tissue processor. I would be interested to hear from people who has had experience with microwave tissue processing. And what brands would you recommend? Thanks a lot, Lucy _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This message is confidential, intended only for the named recipient(s) and may contain information that is privileged or exempt from disclosure under applicable law. If you are not the intended recipient(s), you are notified that the dissemination, distribution, or copying of this message is strictly prohibited. If you receive this message in error, or are not the named recipient(s), please notify the sender at either the e-mail, fax, address, or telephone number listed above and delete this e-mail from your computer. Thank You. ______________________________________________________________________ ------------------------------ Message: 16 Date: Mon, 26 Apr 2010 11:29:34 -0500 From: "Van Eyck, Deb" <deb.vane...@phci.org> Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? To: <ancillaryp...@mac.com>, "ihcrg Group (E-mail)" <ih...@googlegroups.com>, "histonet netserver" <histonet@lists.utsouthwestern.edu> Message-ID: <37212590e341574b96e6796d27df42dab04...@rwdex3.whs.phci.org> Content-Type: text/plain; charset="US-ASCII" This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb ________________________________ From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of ancillaryp...@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" <jtay...@meriter.com> 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ Message: 17 Date: Mon, 26 Apr 2010 10:46:09 -0600 From: "Patsy Ruegg" <pru...@ihctech.net> Subject: [Histonet] RE: [IHCRG] ER clone 1D5 or SP1 ? To: <deb.vane...@phci.org>, <ancillaryp...@mac.com>, "'ihcrg Group \(E-mail\)'" <ih...@googlegroups.com>, "'histonet netserver'" <histonet@lists.utsouthwestern.edu> Message-ID: <c2a6ac4d0bbf46c580d280d99f2b9...@patsyoffice> Content-Type: text/plain; charset="us-ascii" Did I understand correctly from Dr. Hammond in Florida that the ER ASCO/CAP guidelines extended the fixation time to 72 hours? Are they changing the Her2 guidelines to match the ER? If so, has that happened yet? I have people very anxious to stop having techs work on the weekends to comply with the 48 hour fixation limits. Thank you, Patsy Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 wk email pru...@ihctech.net web site www.ihctech.net This email is confidential and intended solely for the use of the Person(s) ('the intended recipient') to whom it was addressed. Any views or opinions presented are solely those of the author. It may contain information that is privileged & confidential within the meaning of applicable law. Accordingly any dissemination, distribution, copying, or other use of this message, or any of its contents, by any person other than the intended recipient may constitute a breach of civil or criminal law and is strictly prohibited. If you are NOT the intended recipient please contact the sender and dispose of this e-mail as soon as possible. _____ From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of Van Eyck, Deb Sent: Monday, April 26, 2010 10:30 AM To: ancillaryp...@mac.com; ihcrg Group (E-mail); histonet netserver Subject: RE: [IHCRG] ER clone 1D5 or SP1 ? This is a great discussion lets also talk about PR clones since the ASCO/CAP guidelines just came out ------Hadi or Rich I know they only list two PR clones one is Dako 1294-----what is the other 312? Deb _____ From: ih...@googlegroups.com [mailto:ih...@googlegroups.com] On Behalf Of ancillaryp...@mac.com Sent: Sunday, April 25, 2010 7:36 PM To: ihcrg Group (E-mail); histonet netserver Subject: Re: [IHCRG] ER clone 1D5 or SP1 ? When we started our lab 3 years ago, we began with SP1 from day 1, so I don't have any experience with either 1D5 or 6F11 except in my previous labs. 1D5 is an excellent clone, and seems to be more specific than SP1 in the work-up of metastatic carcinoma of unknown primary site, based on the published literature. The advantage of 6F11 is that, for those of us who use the Allred scoring system, it's the only clone that was clinically validated by Harvey et al. (JCO 1999) for this purpose. I agree with Rich. For those who use SP1, it's a very good clone as a predictive marker in breast cancer. But again, in the setting of metastatic workup, it is NOT recommended, as it will pick up too many primary lung cancers and some colon cancers (personal experience). Hadi ================================ Hadi Yaziji, M.D., Medical Director Vitro Molecular Laboratories President, Ancillary Pathways 7000 62nd Avenue, PH-C Miami, FL 33143 T 305-740-4440 F. 786-513-0175 www.vitromolecular.com www.ancillarypath.com On Apr 25, 2010, at 3:04 PM, Richard Cartun wrote: I have looked at several clones over the years and I prefer clone 6F11. Richard Richard W. Cartun, Ph.D. Director, Histology & Immunopathology Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital 80 Seymour Street Hartford, CT 06102 (860) 545-1596 Office (860) 545-2204 Fax "Taylor, Jean" <jtay...@meriter.com> 4/23/2010 11:17 AM >>> I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -- Subscription settings: http://groups.google.com/group/ihcrg/subscribe?hl=en This information is confidential and intended solely for the use of the individual or entity to whom it is addressed. If you have received this email in error please notify the sender or our Customer Support Center at (262) 928-2777. We have scanned this e-mail and its attachments for malicious content. However, the recipient should check this email and any attachments for the presence of viruses. ProHealth Care accepts no liability for any damage caused by any virus transmitted by this email. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 77, Issue 32 **************************************** mail2.pph.org made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. 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