Cracks and fissues is not freezing artifact, as from freezing too slow. You are cutting the tissue too cold, and/or the blade angle is too steep. Cordially, Charles W. Scouten, Ph.D Product Manager, MNL Biosystems Division Leica Biosystems Richmond, Inc. 5205 Route 12 P.O. Box 528 Richmond, IL 60071 United States of America Telephone 630 964 0501 facsimile +1 630 964 0576 www.MyNeuroLab.com <http://www.myneurolab.com/> www.leica-microsystems.com <http://www.leica-microsystems.com/> IMPORTANT - This email and any attachments may be confidential. Any retransmissions, dissemination or other use of these materials by persons or entities other than the intended recipient is prohibited. If received in error, please contact us and delete all copies. Before opening or using attachments, check them for viruses and defects. Our liability is limited to resupplying any affected attachments. [Any representations or opinions expressed in this email are those of the individual sender]. From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Andrew Burgeson <nap...@siscom.net> Sent: Monday, May 03, 2010 2:32 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] mouse kidney frozen sectioning Having trouble with freezing artifact in the form of tiny fissures or cracks in mouse kidney on frozen section.
Tissue is paraformaldehyde fixed and infiltrated w 70% aqueous sucrose OCT solution. Anyone else seen this and know how to deal with it? Thx _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet