Erin, Holding the can too close and/or spraying for a prolonged period both cause freeze artifact. Look at your block, it will not have a smooth look to the paraffin. it will have lines it much like a cracked piece of glass. the tissue will appear cracked on the waterbath and will even separate along these lines. The cracks can be seen microscopically amd interfere with the architecture of the tissue. Rena Fail
----- Original Message ---- From: "Martin, Erin" <erin.mar...@ucsf.edu> To: histonet <histonet@lists.utsouthwestern.edu> Sent: Wed, May 19, 2010 12:09:22 PM Subject: [Histonet] Freezing spray artifact Has anyone run into a problem or artifact from freezing spray? I think we may be having a problem with it but I can't find any pictures or descriptions of what it looks like. Thanks in advance, Erin Erin Martin, Histology Supervisor UCSF Department of Dermatopathology 415-353-7248 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
