I just realized the question came from Belgium. I have no idea how they do things there.
Mark On Thu, May 20, 2010 at 2:14 PM, Mark Tarango <marktara...@gmail.com> wrote: > Yes, you can go up to 72 hours for ER/PR (new CAP/ASCO guidlines), but if > you do HER2 the maximum is still 48 hours. I'm assuming you want HER2 as > well, so your best option would probably be to hold the tissues in 70% > alcochol on the processer until Sunday night. > > Mark Tarango > On Thu, May 20, 2010 at 12:54 PM, Liz Chlipala <l...@premierlab.com>wrote: > >> I was talking to Peggy Wenk over the weekend at the MSH meeting and they >> had a paper that was published regarding fixation and ER/PR staining >> sensitivity etc. The biggest problem that they reported is >> underfixation is much worse than over fixation. I think a minimum of 10 >> hours of fixation demonstrated good results and that intensity of >> staining started to decrease but not by much at 48 hours. I would be >> more concerned over underfixation than overfixation. Also the new ER/PR >> guidelines state its acceptable to have samples in fixative for 72 >> hours. Maybe Peggy can post the link to this paper. >> >> Liz >> >> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC >> Manager >> Premier Laboratory, LLC >> PO Box 18592 >> Boulder, Colorado 80308 >> office (303) 682-3949 >> fax (303) 682-9060 >> www.premierlab.com >> >> >> Ship to Address: >> 1567 Skyway Drive, Unit E >> Longmont, Colorado 80504 >> >> -----Original Message----- >> From: histonet-boun...@lists.utsouthwestern.edu >> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA >> MARGRAF >> Sent: Thursday, May 20, 2010 1:04 PM >> To: histonet@lists.utsouthwestern.edu >> Subject: [Histonet] weekend fixation >> >> >> Histonetters: >> Here's a message I was asked to post...... >> >> Dear Colleagues, >> I have the following question concerning tissue processing. We do a >> lot of IHC work on NF fixed tissue. To standardize and minimize the >> effect of NF fixation, we fixate the tissue always for 24h. This is of >> course a problem for tissues taken on Friday. In the past, we asked our >> technicians to come on Saturday to embed the tissues in paraffin. >> Unfortunately, this is not possible anymore, and that is why I need your >> advice. What would you suggest ? 1) to leave the tissue in NF until >> Sunday evening and start processing, or 2) to keep the fixation time (24 >> hours) and leave the tissue in alcohol 70% until Sunday evening and then >> start processing. >> Thanks for your advice. >> Kind regards, >> Wim. >> >> >> Prof. dr. Wim Van den Broeck, DVM, MSc, PhD >> Cell biology and Histology >> Department of Morphology - Faculty of Veterinary Medicine >> Ghent University >> Salisburylaan 133, B-9820 Merelbeke, BELGIUM >> >> >> >> Please consider the environment before printing this e-mail. >> >> This e-mail, facsimile, or letter and any files or attachments >> transmitted with it contains >> information that is confidential and privileged. This information is >> intended only for the >> use of the individual(s) and entity(ies) to whom it is addressed. If you >> are the intended >> recipient, further disclosures are prohibited without proper >> authorization. If you are not >> the intended recipient, any disclosure, copying, printing, or use of >> this information is >> strictly prohibited and possibly a violation of federal or state law and >> regulations. If you >> have received this information in error, please notify Children's >> Medical Center Dallas >> immediately at 214-456-4444 or via e-mail at priv...@childrens.com. >> Children's Medical >> Center Dallas and its affiliates hereby claim all applicable privileges >> related to this >> information. >> >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet