The problem with all this is that the ER/PR fixation times and the her2 fixation times do not match 72 hrs vs 48 hrs. Many times the same specimen that receives ER/PR also gets her2. This is where things are missed up. Why would they ever change one and not the othe...@#$%^&*
Mike -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Liz Chlipala Sent: Thursday, May 20, 2010 2:54 PM To: LINDA MARGRAF; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] weekend fixation I was talking to Peggy Wenk over the weekend at the MSH meeting and they had a paper that was published regarding fixation and ER/PR staining sensitivity etc. The biggest problem that they reported is underfixation is much worse than over fixation. I think a minimum of 10 hours of fixation demonstrated good results and that intensity of staining started to decrease but not by much at 48 hours. I would be more concerned over underfixation than overfixation. Also the new ER/PR guidelines state its acceptable to have samples in fixative for 72 hours. Maybe Peggy can post the link to this paper. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of LINDA MARGRAF Sent: Thursday, May 20, 2010 1:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] weekend fixation Histonetters: Here's a message I was asked to post...... Dear Colleagues, I have the following question concerning tissue processing. We do a lot of IHC work on NF fixed tissue. To standardize and minimize the effect of NF fixation, we fixate the tissue always for 24h. This is of course a problem for tissues taken on Friday. In the past, we asked our technicians to come on Saturday to embed the tissues in paraffin. Unfortunately, this is not possible anymore, and that is why I need your advice. What would you suggest ? 1) to leave the tissue in NF until Sunday evening and start processing, or 2) to keep the fixation time (24 hours) and leave the tissue in alcohol 70% until Sunday evening and then start processing. Thanks for your advice. Kind regards, Wim. Prof. dr. Wim Van den Broeck, DVM, MSc, PhD Cell biology and Histology Department of Morphology - Faculty of Veterinary Medicine Ghent University Salisburylaan 133, B-9820 Merelbeke, BELGIUM Please consider the environment before printing this e-mail. This e-mail, facsimile, or letter and any files or attachments transmitted with it contains information that is confidential and privileged. This information is intended only for the use of the individual(s) and entity(ies) to whom it is addressed. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient, any disclosure, copying, printing, or use of this information is strictly prohibited and possibly a violation of federal or state law and regulations. If you have received this information in error, please notify Children's Medical Center Dallas immediately at 214-456-4444 or via e-mail at priv...@childrens.com. Children's Medical Center Dallas and its affiliates hereby claim all applicable privileges related to this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet