Hi, I am having some trouble with Nissl staining newborn (P0) mouse heads/hindbrain. The nissl stain appears very faint in the centre of my sections. (Paraffin embedded, 7um thick)
Is this likely to be a penetration issue? I fixed in 4% PFA O/N before processing - Would that affect it? I left the skull intact, but removed the skin - should I have removed some of the skull? Any help appreciated!!!!!! Regards, -- Kevin _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
