Did you decal the skull? Sarah Goebel, B.A., HT (ASCP)
Histotechnician XBiotech USA Inc. 8201 Eas= t Riverside Dr. Bldg 4 Suite 100 = Austin, Texas 78744 (512)386-5107 -------- Original Message -------- Subject: [Histonet] Nissl Stain P0 Mouse Head From: Kevin Gillinder <[1]= k.r.gillin...@newcastle.ac.uk> Date: Fri, May 21, 2010 5:00 am To: "Histonet@lists.utsouthwestern.edu" <[2]histo...@lists.uts= outhwestern.edu> Hi, I am having some trouble with Nissl staining newborn (P0) mouse heads/hindb= rain. The nissl stain appears very faint in the centre of my sections. (Paraffin = embedded, 7um thick) Is this likely to be a penetration issue? I fixed in 4% PFA O/N before processing - Would that affect it? I left the skull intact, but removed the skin - should I have removed some = of the skull? Any help appreciated!!!!!! Regards, -- Kevin _______________________________________________ Histonet mailing list [3]histo...@lists.utsouth= western.edu [4]http://lists.utsouthwestern.edu/mailman/listinfo/histonet<= br> References 1. 3D"mailto:k.r.gillin...@newcastle.ac.uk"; 2. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 3. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 4. 3D"http://lists.utsouthwestern.edu/mailman/listin_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
