** Proprietary ** ** Reply Requested When Convenient ** Dear all,
Lately we have been experiencing problem with our Retic Stains, who keep lifting off slides after the gold chloride step. We have changed all solutions one by one, trying to see which one was causing problem without success. BMT Bx are cut at 3 micron on X-tra adhesive slides. BMT BX are decal in 10 % Formic Formaldehyde. Here is the protocol that we are currently using. 1. Sections to water. 2. Treat with 0.25% acidified Potassium permanganate. --- 5 mins 3. Bleach in oxalic acid. 4. Rinse in distilled water. 5. Treat with 5% iron alum. --- 15 mins 6. Rinse briefly in distilled water. 7. Impregnate with filtered ammoniacal silver solution ---1 min NB: Time in ammoniacal silver may need to be increased up to 5 min for bone marrow trephines 8. Rinse well in distilled water. 9. Reduce in 10% formalin (in tap water). --- 1min 10. Rinse in distilled water. 11. Tone in 0.2% Gold Chloride ---1 min 12. Rinse in distilled water. 13. Fix in 5% sodium thiosulphate (hypo) --- 30 sec 14. Wash in water. 15. Counterstain with neutral red. 16. Dehydrate, clear and mount. Any suggestions would be appreciated. Malika Malika Benatti Specialist BMS Camelia Botnar Laboratories Histopathology Department Great Ormond Street Hospital London WC1N 3JH Tel: +44 20 7405 9200 ext 5475 Fax: +44 20 7829 7875 ben...@gosh.nhs.uk ********************************************************************************************************* This message may contain confidential information. If you are not the intended recipient please inform the sender that you have received the message in error before deleting it. Please do not disclose, copy or distribute information in this e-mail or take any action in reliance on its contents: to do so is strictly prohibited and may be unlawful. Thank you for your co-operation. ********************************************************************************************************* _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
