We use the AmericanMasterTech Alcian Blue pH 2.5 and use this procedure: Place slides in 3% Acetic Acid for 3 minutes. Place slides in Alcian Blue for 30 minutes. Rinse slides in water Place slides in 0.5% Periodic Acid for 10 minutes. Rinse slides in water Place slides in Schiff Reagent for 15 minutes Rinse slides in HOT water for 5 minutes. Counter stain in Hematoxylin for 2 minutes. Rinse slides in water, Dehydrate slides through Absolute Alcohol, Clear through Xylene
Katelin Lester, HTL(ASCP) MedSurg Pathology Associates, Inc. (503)443-2157 > Can anyone share with me their procedure for AB-PAS for Barrett's > Esophagus. > With thanks > > Diana > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet