Sorry, I forgot about attachments. Here is the procedure. REPROCESSING DRIED OR SUB-OPTIMAL TISSUE SPECIMENS
PURPOSE: This procedure provides instructions for rehydrating dried tissue specimens. PRINCIPLE: Tissue may become dry due to a malfunctioning of the processor, or if the tissue has inadvertently been left out of fixative for an extended period of time. The fixative can also sometimes evaporate from the container, or leak out if the lid is not tightly sealed. Some cadaver tissues may be dried out if the body is discovered some days after death has occurred and been exposed to the elements. TEST METHOD INSTRUCTIONS: N/A SPECIMEN TYPE/: Any tissue that is dried or brittle either before or after fixation, or improperly processed paraffin blocks resulting from processor malfunction. EQUIPMENT: Tissue processor (VIP) Graduated cylinders Metal tissue molds Glass containers SAFETY REQUIREMENTS/SPECIAL HANDLING: When reprocessing tissue specimens, gloves, goggles, and a plastic apron or laboratory coat should be worn. Formalin is a suspected carcinogen. Glycerin should be handled under a hood. REAGENTS NEEDED: Formol- Sodium Acetate (Stock) Solution Formol- Glycerol (Working) Solution Paraffin Xylene 100% Dehydrant 95% Dehydrant REAGENT PREPARATION: Formol-Sodium Acetate (Stock) Solution Concentrated formalin (37%) solution____________________50.0 ml Sodium Acetate______________________________________10.0 g Tap water___________________________________________450.0 ml Formol-Glycerol (Working) Solution Formol-Sodium Acetate (Stock) Solution__________________450.0 ml Glycerol (glycerin)____________________________________50.0 ml PROCEDURE: A. If tissue is dry due to a malfunction of the processor, follow steps 6-7 of the reprocessing schedule below. B. If the tissue is left out of fixative for a period of time, or if the fixative evaporates, follow steps 6-7 of the reprocessing schedule below. C. If at microtomy, paraffin blocks do not section due to improper impregnation with paraffin, reprocessing can be accomplished by following all steps of the reprocessing schedule. Reprocessing Schedule: 1. Place paraffin blocks in molten paraffin wax (60 C) for two (2) hours. 2. Remove tissue from paraffin and place in three (3) changes of xylene for one (1) hour each. 3. Place tissue in 100% dehydrant, two (2) changes, for one (1) hour each. 4. Place tissue in 95% dehydrant, two (2) changes, for one (1) hour each. 5. Place tissue under running tap water for 20 minutes. 6. Place tissue in formol-glycerol working solution until softening occurs. (This usually occurs within 5-8 hours. Extended exposure to the formol-glycerol solution will not harm tissue.) 7. Place tissue on the processor and proceed with routine processing starting in the dehydrant. (It is not necessary to expose the tissue to further fixation.) EXPECTED RESULTS: Dried tissue specimens are softened and acceptable histologic preparations can then be generated. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan Sent: Thursday, July 15, 2010 9:58 AM To: Carol Fields; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] FW: Fried biopsies This is a procedure that I have used in the past and it has worked very well. Good luck! Kaye -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carol Fields Sent: Thursday, July 15, 2010 9:43 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] FW: Fried biopsies From: Carol Fields Sent: Thursday, July 15, 2010 9:37 AM To: 'histonet-boun...@lists.utsouthwestern.edu' Subject: Fried biopsies Hi Histotechs, We have fried GI biopsies this morning and I remember a solution you can soak them in to reprocess them and hopefully make them diagnosable. It has been several years since I used this solution so I do not have the recipe. Does anyone out there ....Derm lab, or anywhere that knows how to back this tissue up to salvage it? Any help with this is greatly appreciated. Carole Carole Fields, HT (ASCP) Histology Supervisor Northside Hospital Atlanta, GA 30342 carol.fie...@northside.com CONFIDENTIALITY NOTICE: This electronic mail transmission has been sent by Northside Hospital. It may contain information that is confidential, privileged, proprietary, or otherwise legally exempt from disclosure. If you are not the intended recipient, you are hereby notified that you are not authorized to read, print, retain, copy or disseminate this message, any part of it, or any attachments. If you have received this message in error, please delete this message and any attachments from your system without reading the content and notify the sender immediately of the inadvertent transmission. There is no intent on the part of the sender to waive any privilege. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet