Hello Histonetters, I am working with archaeological residues lifted from Holocene grindstones. I was trying to find a stain that could differentiate between plant and animal tissue in one hit. I have been using buffered Toluidine Blue solutions however given that these are ancient residues which are lifted using 20ul of water, the subtle colour differences between blues, purples, and violets have not been useful. I was looking at the possibility of counterstaining the Toluidine Blue stained residues with say Phloroglucinol or IKI which would highlight the plant component of the residue and then see if what was left looked like collagen. Has anyone tried this type of counterstaining? OR does anyone know of a stain that would colour differently for plant and animals understanding that this is not a tissue section but microscopic residues in solution? Thanks Birgitta Stephenson The Research Microscopy Laboratory, University of Queensland. -- Birgitta Stephenson bstep...@fastmail.fm
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