Yes, ORO stains lipids but I don't think it would work for crude. Would you look for artifact and tissue reaction? Pam Vlies, HT-ASCP Waukegan IL Sent on the Sprint® Now Network from my BlackBerry®
-----Original Message----- From: histonet-requ...@lists.utsouthwestern.edu Sender: histonet-boun...@lists.utsouthwestern.edu To: <histonet@lists.utsouthwestern.edu> Reply-To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 80, Issue 34 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Gordon and Sweets retic stain (Johnson, Nacaela) 2. RE: Histonet Digest, Vol 80, Issue 33 (Joanne Clark) 3. Sakura VIP6 update (Cynthia Pyse) 4. dissection aide (Tench, Bill) 5. Refrigerate formalin? (Breeden, Sara) 6. Re: Refrigerate formalin? (Rene J Buesa) 7. REFRIG FORMALIN (Tench, Bill) 8. Re: Refrigerate formalin? (Mark Ray) 9. Re: ? on Gulf oil spill (Lesley Weston) 10. PECAM-1 (sc-1506R) staining (Victor Wong) 11. RE: Refrigerate formalin? (Johnson, Nacaela) 12. NEW Opportunity with a World Leader in IHC in New England! (Matthew Ward) 13. Re: Refrigerate formalin? (Geoff McAuliffe) 14. FAST interpretation (sarah Tabatabaei) 15. Cool Formalin (Breeden, Sara) 16. plastics (Dixon,Maryann) 17. knife holder (Dixon,Maryann) 18. p53 IHC staining in mouse (Ross Benik) ---------------------------------------------------------------------- Message: 1 Date: Wed, 28 Jul 2010 12:11:42 -0500 From: "Johnson, Nacaela" <nacaela.john...@usoncology.com> Subject: [Histonet] Gordon and Sweets retic stain To: <histonet@lists.utsouthwestern.edu> Message-ID: <6dbd71c31d7e444482e5d3dfbc202d260245d...@txhous1eb012.uson.usoncology.int> Content-Type: text/plain; charset="us-ascii" Hello! I am having a problem with my tissue washing off of the slides during the retic stain. The majority of the tissue is falling off during the Working silver solution step because of the alkalinity of the solution. The tissue is bone marrow and I use Halt in my water bath. My thoughts are to add a mild acid to the working solution to bring the pH down, but I am not sure of what the effects are on the solution. I do not want the silver to not impregnate. Has anyone had this problem before and found a solution? Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com </pre>The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.<br>Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone.</pre> ------------------------------ Message: 2 Date: Wed, 28 Jul 2010 12:34:49 -0600 From: "Joanne Clark" <jcl...@pcnm.com> Subject: [Histonet] RE: Histonet Digest, Vol 80, Issue 33 To: <histonet@lists.utsouthwestern.edu> Message-ID: <0cda5e1e01301f4880a8a7a8bcbda39c012f7...@mail.pcnm.com> Content-Type: text/plain; charset="iso-8859-1" We use a similar product called Dissect Aid, from Decal Corp. IHC staining has never been an issue; we never run IHC on the lymph nodes from colon cancer cases. We usually do the IHC on the tumor itself which is fixed in formalin. This kind of product is very useful in helping to find all those pesky little nodes that like to hide in the fat by turning them white. Cancer protocols require that at least 13 nodes be submitted. As histo supervisor, I also do the grossing in my facility, so I understand why your PA is interested in this kind of product (it can be difficult to come up with this magic number of 13 without it). However, if you do run IHC on the nodes from these cases, you would indeed have to revalidate your markers with this as your primary fixative or as a post fixative. Joanne Clark, HT, MLT Histology Supervisor Pathology Consultants of New Mexico Roswell, NM ----------------------------- Message: 2 Date: Tue, 27 Jul 2010 15:03:33 -0300 From: "Hayes, Randi (HorizonNB)" <randi.ha...@horizonnb.ca> Subject: [Histonet] Using GEWF solution and IHC staining To: <histonet@lists.utsouthwestern.edu> Message-ID: <c2889f00e87e8d4ea5798737d8f7f362a73...@rhaex1.rha-rrs.ca> Content-Type: text/plain; charset="iso-8859-1" At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how "safe" it is to use when staining for IHC. Does anyone have much experience with this or know of a study (studies) that have been done to verify that the ethanol is not destroying antigen sites? We're a little concerned...... Randi Hayes, MLT Histology Supervisor / Superviseur d'Histologie Horizon Health Network / Réseau de santé Horizon (506) 860-2157 randi.ha...@horizonnb.ca <http://www.me.com/mail/> www.HorizonNB.ca <http://www.horizonnb.ca/> ------------------------------ Message: 3 Date: Wed, 28 Jul 2010 14:40:21 -0400 From: "Cynthia Pyse" <cp...@x-celllab.com> Subject: [Histonet] Sakura VIP6 update To: <histonet@lists.utsouthwestern.edu> Message-ID: <002101cb2e84$55ced450$016c7c...@com> Content-Type: text/plain; charset="us-ascii" Thanks everyone for your responses. Sakura has scheduled an upgrade of my rotary and gate valve. I hope this is the answer I need. Cindy ------------------------------ Message: 4 Date: Wed, 28 Jul 2010 12:12:30 -0700 From: "Tench, Bill" <bill.te...@pph.org> Subject: [Histonet] dissection aide To: histonet@lists.utsouthwestern.edu Message-ID: <2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local> Content-Type: text/plain; charset=us-ascii I don't see much advantage of this over plain old Carnoy's fixative, other than missing the wiff of choroform. Ethanol should not be a problem with IHC, but as you said, revalidation is required. The magic number of nodes is 12. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- ------------------------------ Message: 5 Date: Wed, 28 Jul 2010 14:41:04 -0600 From: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Subject: [Histonet] Refrigerate formalin? To: <histonet@lists.utsouthwestern.edu> Message-ID: <4d14f0fc9316dd41972d5f03c070908b02e47...@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ------------------------------ Message: 6 Date: Wed, 28 Jul 2010 13:49:52 -0700 (PDT) From: Rene J Buesa <rjbu...@yahoo.com> Subject: Re: [Histonet] Refrigerate formalin? To: histonet@lists.utsouthwestern.edu, SaraBreeden <sbree...@nmda.nmsu.edu> Message-ID: <304094.35306...@web65715.mail.ac4.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 There is nothing against cooling formalin fixed tissues, but my question is WHY? I see it as a waste of refrigerated space. On the other hand also, IF by any change there is a formalin spill inside the walk-in cooler room, that will be a royal mess to decontaminate. René J. --- On Wed, 7/28/10, Breeden, Sara <sbree...@nmda.nmsu.edu> wrote: From: Breeden, Sara <sbree...@nmda.nmsu.edu> Subject: [Histonet] Refrigerate formalin? To: histonet@lists.utsouthwestern.edu Date: Wednesday, July 28, 2010, 4:41 PM We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Wed, 28 Jul 2010 13:50:28 -0700 From: "Tench, Bill" <bill.te...@pph.org> Subject: [Histonet] REFRIG FORMALIN To: histonet@lists.utsouthwestern.edu Message-ID: <2820431bf953bb4da3e9e1a5882265fd034a5...@mail1.pph.local> Content-Type: text/plain; charset=us-ascii You must have a heck of a lot of space in the frig. I am envious. I don't think refrigerating your specimens would create any problems. Everyone i know stores them in some inconvenient corner of a morgue at room temperature. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- NOTICE: This email message is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply email and destroy all copies of the original message. This message has been content scanned by the Axway MailGate. MailGate uses policy enforcement to scan for known viruses, spam, undesirable content and malicious code. For more information on Axway products please visit www.axway.com. --------------------------------------------------------------------- ------------------------------ Message: 8 Date: Wed, 28 Jul 2010 16:24:37 -0500 From: Mark Ray <darkd...@comcast.net> Subject: Re: [Histonet] Refrigerate formalin? To: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Cc: histonet@lists.utsouthwestern.edu Message-ID: <4c50a015.40...@comcast.net> Content-Type: text/plain; charset=ISO-8859-1; format=flowed It is usually recommended that 10% Formalin Fixative be stored at temperatures above about 10C to minimize polymerization of Formaldehyde. You might confirm this with the manufacturer of your Formalin Fixative. The label may also say something about this. Breeden, Sara wrote: > We're getting ready to move into our new building (YAHOO!) in early > September - finally. In planning where we will store our cases for the > required 6-week retention time, I have proposed that the tissues (in 10% > NBF) be shelved in the walk-in cooler (4 degrees C). Is there any > reason tissues-in-formalin could NOT be stored refrigerated for that > length of time? It is extremely rare that we are required to pull and > recut wet tissue, but that possibility always exists. Thanks, everyone. > > > > Sally Breeden, HT(ASCP) > > Veterinary Diagnostic Services > > New Mexico Department of Agriculture > > 700 Camino de Salud NE > > Albuquerque, NM 87108 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ------------------------------ Message: 9 Date: Wed, 28 Jul 2010 15:54:18 -0700 From: Lesley Weston <les...@shaw.ca> Subject: Re: [Histonet] ? on Gulf oil spill To: jstaruk <jsta...@masshistology.com> Cc: histonet@lists.utsouthwestern.edu Message-ID: <378ea25a-d24e-4961-9684-6370b09bc...@shaw.ca> Content-Type: text/plain; charset=us-ascii Oil Red O might work, since it stains lipids. Lesley Weston. On 2010-07-28, at 7:26 AM, jstaruk wrote: > Hi all, > > I have a customer who wants to study the amounts of oil accumulation in the > gills of fish from the Gulf of Mexico. Does anyone have any suggestions as > to what techniques might stain crude oil? > > Thanks > > Jim > > _______________________ > James E. Staruk HT(ASCP) > www.masshistology.com > www.nehorselabs.com > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 10 Date: Wed, 28 Jul 2010 21:05:45 -0700 (PDT) From: Victor Wong <vhlw...@yahoo.com> Subject: [Histonet] PECAM-1 (sc-1506R) staining To: histonet@lists.utsouthwestern.edu Message-ID: <983283.48384...@web52701.mail.re2.yahoo.com> Content-Type: text/plain; charset=iso-8859-1 Dear all, Thank you for all the professional suggestion of the previous IHC staining and finally I got some positive results. Then comes problem on the PECAM-1 IHC. I am using the Santa Cruz sc-1506R (it is a anti-RAT antibody from RABBIT). I stain decalcified rat spine paraffin sections, with following steps: antigen retrieval (~95C MW, DAKO AR solution pH6 for 20 minutes); DAKO's Dual Enzyme block for 10 minutes; 3% normal goat serum for 20 minutes; 1:50 primary antibody at 4C for overnight; DAKO's Envision anti-rabbit kit for 30 minutes; DAKO's DAB solution to develop I used rat liver and kidney as positive controls. I got background staining on hepatocytes but none staining on vessels. On kidney section, nucleus and lining within tubules and basement membrane of glomerulus were also stained but not that bad as liver. I cannot say it was totally non-specific as some nucleus were not stained. I think PECAM-1 IHC needs more specific conditons. Could any histoneters have any suggestion? Many thanks in advance. Victor ------------------------------ Message: 11 Date: Thu, 29 Jul 2010 07:49:02 -0500 From: "Johnson, Nacaela" <nacaela.john...@usoncology.com> Subject: RE: [Histonet] Refrigerate formalin? To: "Breeden, Sara" <sbree...@nmda.nmsu.edu>, <histonet@lists.utsouthwestern.edu> Message-ID: <6dbd71c31d7e444482e5d3dfbc202d260245d...@txhous1eb012.uson.usoncology.int> Content-Type: text/plain; charset="us-ascii" I had a retic kit that was put in the refrigerator by one of my collegues. The formalin was stated to be stored at room temp, but was left in the box. The cold temperature broke the formalin down and it no longer worked to reduce the silver. My suggestion would be to keep them at room temperature to be on the safe side. Thanks, Nacaela Johnson Histology Technician KCCC Pathology 12000 110th St., Ste. 400 Overland Park, KS 66210 Office: 913-234-0576 Fax: 913-433-7639 Email: nacaela.john...@usoncology.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Breeden, Sara Sent: Wednesday, July 28, 2010 3:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Refrigerate formalin? We're getting ready to move into our new building (YAHOO!) in early September - finally. In planning where we will store our cases for the required 6-week retention time, I have proposed that the tissues (in 10% NBF) be shelved in the walk-in cooler (4 degrees C). Is there any reason tissues-in-formalin could NOT be stored refrigerated for that length of time? It is extremely rare that we are required to pull and recut wet tissue, but that possibility always exists. Thanks, everyone. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet </pre>The contents of this electronic mail message and any attachments are confidential, possibly privileged and intended for the addressee(s) only.<br>Only the addressee(s) may read, disseminate, retain or otherwise use this message. If received in error, please immediately inform the sender and then delete this message without disclosing its contents to anyone.</pre> ------------------------------ Message: 12 Date: Thu, 29 Jul 2010 09:15:03 -0400 From: "Matthew Ward" <m...@personifysearch.com> Subject: [Histonet] NEW Opportunity with a World Leader in IHC in New England! To: <histonet@lists.utsouthwestern.edu> Message-ID: <010f01cb2f20$0eb960c0$2c2c22...@com> Content-Type: text/plain; charset="us-ascii" Good Morning All, Our team at Personify is currently partnered with a World Leader in IHC that is currently looking for a Field Support Specialist in the New England area. This is the perfect opportunity for a histotech to move off the bench and get into the field on the manufacturers side! The position offers the following: Base Salary! Bonus! Car Allowance and Paid Expenses! Outstanding Benefits! Opportunity for advancement! Please contact me immediately to learn more! Matt Ward Account Executive Personify 201 Shannon Oaks Circle, Suite 101 Cary, North Carolina 27511 (Tel) 800.875.6188 direct ext 103 (Fax) 919.460.0642 <http://www.personifysearch.com/> www.personifysearch.com ------------------------------ Message: 13 Date: Thu, 29 Jul 2010 09:47:59 -0400 From: Geoff McAuliffe <mcaul...@umdnj.edu> Subject: Re: [Histonet] Refrigerate formalin? To: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Cc: histonet@lists.utsouthwestern.edu Message-ID: <4c51868f.90...@umdnj.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed I agree with Rene and Mark. Geoff Breeden, Sara wrote: > We're getting ready to move into our new building (YAHOO!) in early > September - finally. In planning where we will store our cases for the > required 6-week retention time, I have proposed that the tissues (in 10% > NBF) be shelved in the walk-in cooler (4 degrees C). Is there any > reason tissues-in-formalin could NOT be stored refrigerated for that > length of time? It is extremely rare that we are required to pull and > recut wet tissue, but that possibility always exists. Thanks, everyone. > > > > Sally Breeden, HT(ASCP) > > Veterinary Diagnostic Services > > New Mexico Department of Agriculture > > 700 Camino de Salud NE > > Albuquerque, NM 87108 > > 505-841-2576 > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 mcaul...@umdnj.edu ********************************************** ------------------------------ Message: 14 Date: Thu, 29 Jul 2010 07:04:29 -0700 (PDT) From: sarah Tabatabaei <sarah_t...@yahoo.com> Subject: [Histonet] FAST interpretation To: histonet@lists.utsouthwestern.edu Message-ID: <395564.60128...@web45707.mail.sp1.yahoo.com> Content-Type: text/plain; charset=us-ascii Hi, I'm using FAST profile (Fast green, Alcian Blue, Safranin-O, and Tartrazine) to stain my human Intervertebral Discs. how can I figure out which color resembles which type of tissue? Regards -Sarah ------------------------------ Message: 15 Date: Thu, 29 Jul 2010 09:04:46 -0600 From: "Breeden, Sara" <sbree...@nmda.nmsu.edu> Subject: [Histonet] Cool Formalin To: <histonet@lists.utsouthwestern.edu> Message-ID: <4d14f0fc9316dd41972d5f03c070908b02e47...@nmdamailsvr.nmda.ad.nmsu.edu> Content-Type: text/plain; charset="us-ascii" Thanks to everyone that replied to my question about refrigerating 10% NBF for up to 6 weeks. I'll fold your information into the decision. Sally Breeden, HT(ASCP) Veterinary Diagnostic Services New Mexico Department of Agriculture 700 Camino de Salud NE Albuquerque, NM 87108 505-841-2576 ------------------------------ Message: 16 Date: Thu, 29 Jul 2010 11:34:43 -0400 From: "Dixon,Maryann" <dix...@ufl.edu> Subject: [Histonet] plastics To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fa9822f11a2edb4eabeb80dfa3534b970555f...@hsc-cms01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Hi histoland, Thinking of getting into plastics and need to know information, pros/cons about them. I've never worked with anything else except paraffin. I will be embedding mostly osteosarcomas. Can someone please give me a call or email me. All suggestions are very appreciated. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dix...@ufl.edu ------------------------------ Message: 17 Date: Thu, 29 Jul 2010 12:54:51 -0400 From: "Dixon,Maryann" <dix...@ufl.edu> Subject: [Histonet] knife holder To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <fa9822f11a2edb4eabeb80dfa3534b970555f...@hsc-cms01.ad.ufl.edu> Content-Type: text/plain; charset="us-ascii" Greetings histonetters!!! Does anyone out there have a paraffin knife holder (knife holder B) for a Leica SM2500 polycut microtome that you might like to part with. I am in need of finding one. Thank you. MaryAnn Dixon BS, HT (ASCP)cm Biological Scientist Surgical Oncology UF College of Veterinary Medicine Phone (352) 294-4516 Email: dix...@ufl.edu ------------------------------ Message: 18 Date: Thu, 29 Jul 2010 10:56:56 -0600 From: "Ross Benik" <r...@premierlab.com> Subject: [Histonet] p53 IHC staining in mouse To: <histonet@lists.utsouthwestern.edu> Message-ID: <ee33be5c905a3046a7ff8f58a64c8e4b0a8...@server.premierlab.local> Content-Type: text/plain; charset="us-ascii" Hi everyone, I am trying to accomplish IHC staining for a rabbit anti p53 antibody in mouse tissue however all I am getting is background and IgG staining. I run my human positive control tissue along with the same protocol parameters and the staining is perfect. I have tried two abcam antibodies (ab32049 and ab4060) and both are not working correctly on mouse tissue. Does anyone know of a p53 antibody that is known to work in mouse tissue? Thanks, Ross ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 80, Issue 34 ****************************************
_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet