We don't perfom Masson Trichrome but the one-step Gomori version and another rather similar trichrome called SFOG. I use Bouins for one year, better said, when the reagens in the coplin jar goes low, I fill it up again. Our selfmade SFOG solution is used even longer than one year. And the commercial Gomori-solution is used for a half year. Weigert's is made once a week, but the results show that the older solution doesn't work as well. Since nuclei aren't really important in the trichromes, it doesn't matter that much.
It's important, that the pH is correct and low enough, to render the solution stable. Our numbers of stained trichromes are about 150 to 200 per year. Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Sherwood, Margaret Gesendet: Montag, 09. August 2010 16:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Re: [Special Stains] Masson's Trichrome To all: We keep having the same discussion re: special stains. How long can you use them before discarding? I am specifically referring to the stains used in Masson's Trichrome. We are a research lab and, therefore, don't run the volume that most labs do. We have found, for instance, that we can use the Weigert's Hematoxylin (A&B) more than once, with filtering (even though it is stated it should be made fresh). We also use Bouin's Fix, Biebrich Scarlet and Aniline Blue more than once. Is there a "set rule" when we should discard these solutions (i.e. after 5X use or 1 month)? I would appreciate some feedback from the experts! Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDW 214) Massachusetts General Hospital 55 Fruit Street Boston, Massachusetts 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet