I think tissue shrinkage is a fairy tail, told by pathologists at the grossing. ;) "Sure, it is a little bit large, but it will shrink.." Too big blocks stay too big while embedding.
**just for smiling** There's a study by Cecil Fox 1985 (Formaldehyde fixation), who showed that shrinkage while fixation occurs only with concentrated formaldehyd, perhaps due to the methanol-part in commercial formalin. For dehydration, "de-fattation" and infiltration I think, it depends on the gradationsteps of the solutions, the applied temperature and the "ingredients" of the tissue. With our standard protocol (13 hours VIP) I never found excessiv shrinkage. So, like always, it depends... Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Tench, Bill Gesendet: Dienstag, 24. August 2010 17:33 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] shrinkage I have never seen a study, but when training, we were taught that fixation and processing could result in up to 5-10% shrinkage. Bill Tench Associate Dir. Laboratory Services Chief, Cytology Services Palomar Medical Center 555 E. Valley Parkway Escondido, California 92025 bill.te...@pph.org Voice: 760- 739-3037 Fax: 760-739-2604 [None] made the following annotations --------------------------------------------------------------------- Confidential E-Mail: This e-mail is intended only for the person or entity to which it is addressed, and may contain information that is privileged, confidential, or otherwise protected from disclosure. Dissemination, distribution, or copying of this e-mail or the information herein by anyone other than the intended recipient is prohibited. If you have received this e-mail in error, please notify the sender by reply e-mail, and destroy the original message and all copies. --------------------------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet