We use the modified Steiner - the kit from Sigma,to be precise. Bernice
Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa Sent: Tuesday, August 31, 2010 10:55 AM To: histonet@lists.utsouthwestern.edu; Chakib Boussahmain Subject: Re: [Histonet] Warthin-Starry Stain Because of intrinsic difficulties and no special advantages, I switched many years ago to the Modified-Steiner method, a much simpler and consistent technique. If you don't have some pathologist unreasonably unwilling to accept anything but WS, switch also. I think it is better to have always good results, instead of struggling with a technique that eventually will delay the slides to be presented to the pathologist. René J. --- On Tue, 8/31/10, Chakib Boussahmain <chak_...@yahoo.com> wrote: From: Chakib Boussahmain <chak_...@yahoo.com> Subject: [Histonet] Warthin-Starry Stain To: histonet@lists.utsouthwestern.edu Date: Tuesday, August 31, 2010, 10:22 AM Hi Histonet, Does anyone out there uses Warthin-Starry Stain, If so, Can you help me with troubleshooting, my slides get overstained even though i respect the developing time that is in the procedure which is 3.5min. thank you in advance and your help will be very much appreciated! Chakib HTL(ASCP) MIT _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet