I'm a guest in a lab with very little freezer/fridge space so I'm wondering if I can mount fixed-frozen testes tissue onto slides, and once dry, store in a slide box at room temperature for a few weeks until all my slides are ready for staining. Tissues are fixed in 10% formalin then sunk in 20% sucrose. I'll embed in OCT (optimal cutting temperature compound) before cryosectioning. I will mount them after a rinse in PBS (phosphate buffered solution). I worry about the tissues over drying since they are not embedded in paraffin. Does anyone have any experience with this?
Thanks! -- Teresa Iglesias Graduate Group in Animal Behavior University of California-Davis _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
