Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nuclei good enough. 1) Is there a way to enhance the nuclei staining ? (the nuclei is the only reason that i"m not using the simpler sirius red and fast green staining.) 2) What is the meaning for washing in running tap water washing, is it done by putting the slides in a jar with simple tap water for a few minutes ?
Thank's Itai _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet