We used the same antibody but then at 1:1200, retrieved with PH9 (PT module buffer 4, thermo) on a labvision stainer. We had beautiful results. Now, we switched to Ventana Benchmark XT (1:50, 30' cc1 + amplification) and our results are quite poor for PMS2: many cytoplasmatic staining. So if anybody has a good protocol for the Benchmark XT, I am very interested. Regards, Willem Hoekert OLVG, Amsterdam
________________________________ Van: histonet-boun...@lists.utsouthwestern.edu namens Dana Settembre Verzonden: wo 25-8-2010 12:12 Aan: dianar...@aol.com; histonet@lists.utsouthwestern.edu Onderwerp: Re: [Histonet] PMS2 Diane, When I was doing PMS2 I was using BD's mouse antibody @ 1:10 Retreived with Dako's TRS in a steamer for 40 min, incubated the antibody for 60 minutes and I used a labelled polymer for the detection (Dako's Envision + Mouse) It was difficult to work up. Good Luck, Dana Settembre, HT ASCP Immunohistochemistry Lab UMDNJ - University Hospital Newark, NJ USA >>> <dianar...@aol.com> 08/24/10 8:47 PM >>> Can anyone share their protocol for PMS2? I just keep getting background staining. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Disclaimer: Dit e-mail bericht is uitsluitend bestemd voor de geadresseerde(n). Verstrekking aan en gebruik door anderen dan geadresseerden is niet toegestaan. Indien u niet de geadresseerde bent, wordt u verzocht de verzender hiervan op de hoogte te stellen en het bericht te verwijderen. In verband met electronische verzending kunnen aan dit e-mail bericht geen rechten worden ontleend. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet