First off you shouldn't leave Bouin's sitting for that long out o= n a shelf. Once the water evaporates out of the solution it is extrem ely flammable (explosive even I think?). Also, you shouldn't leave ti= ssue in alcohol forever either because you will end up with "beef jerky" su= per hard tissues. If you want to keep it in alcohol, maybe make up an= alcoholic formalin solution. I would say try to process it if it is = needed (it might be ok?), but if it was me I would probably just chalk it u= p to the tissue is trash now...Good Luck...let me know what happens!!
Sarah= Goebel, B.A., HT (ASCP) Histotechnician XBio= tech USA Inc. 8201 East Riverside Dr. Bldg 4 Suite 100<= /em> Austin, Texas 78744 <= span style="color: rgb(51, 102, 255);">(512)386-5107 <= /div> -------- Original Message -------- Subject: [Histonet] How long ... From: Massimo <[1]max_histo...@yah= oo.it> Date: Sun, September 19, 2010 3:41 pm To: [2]histo...@lists.uts= outhwestern.edu Cc: [3]histo...@lists.uts= outhwestern.edu Hi all, I found into a shelf of the laboratory a small flask containing mouse sampl= es fixed in Bouin's fluid and preserved in ethanol at 70°, forgotten ther= e for a few years. I wonder if it would be possible to continue the process up to paraffin embedding for histological preparations. Some time ago a professor of biology at the University of Florence told me = that they could remain in alcohol for years, but on literature I found that the = time can not be so long without altering tissues. Has anyone had a similar experience? It's better to throw everything away or not? Thank you in advance. Kind Regards, Massimo Tosi _______________________________________________ Histonet mailing list [4]histo...@lists.utsouth= western.edu [5]http:= //lists.utsouthwestern.edu/mailman/listinfo/histonet References 1. 3D"mailto:max_histo...@yahoo.it"; 2. 3D"mailto:histonet@lists.utsouthwestern.edu"; 3. 3D"mailto:histonet@lists.utsouthwestern.edu"; 4. 3D"mailto:Histonet@lists.utsouthwestern.edu"; 5. 3D"http://lists.utsouthwestern.edu/mailman/listinfo/histonet"; _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
