Merced is correct about longer fixation inducing more cross-linking,
possibly making immunostaining more difficult.
Much depends on the antigen you are looking for.
I disagree that the tissue will become brittle, this is not my experience.
Geoff
Salim Yalcin Inan wrote:
Hello,
I have a question about post-fixation. As far as I know, for most of the
immunohistochemistry studies in mouse/rat brain, post-fixation period is
between 2 hours to 24 hours in 4% paraformaldehyde at +4 Celsius followed by
cryoprotection with 20-30% sucrose solution. Are there any disadvantages to
post-fix the tissues for more than 24 hours (2 days-1 week) for further
immunohistochemistry experiments? Thank you very much in advance.
Sincerely Yours,
Salim Inan, PhD
HBI, University of Calgary
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Neuroscience and Cell Biology
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