We were never able to get the TRAP working with Formic Acid decal. Using a home brewed TRAP kit or the Sigma Kit. But Cell Marque has a TRAcP antibody (catalog 341M-95) that is used as a hairy cell marker. But it also stains macrophages and osteoclasts.
Loralee McMahon, HTL (ASCP) Immunohistochemistry Supervisor Strong Memorial Hospital Department of Surgical Pathology (585) 275-7210 ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Praveen Arany [ar...@fas.harvard.edu] Sent: Wednesday, October 06, 2010 9:17 AM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Osteoclast Hi Loralee, I am having similar problems with Formic acid Decal'd sections for TRAP. After EDTA, are you doing a TRAP IHC or the enzyme assay from Sigma? As for molecular markers, Cathepsin K is pretty good for Osteoclasts but I do westerns in a research setting......has anyone had good success with any antibody for immunostaining? Best, Praveen Arany, Graduate Student, Harvard University, Cambridge MA On 10/6/2010 8:43 AM, McMahon, Loralee A wrote: > You can do a TRAP stain on EDTA decaled bone. I haven't been able to get it > to work on formic acid decaled bone. I also believe that there is an > antibody for osteoclasts, but I have not tried it. > > Loralee McMahon, HTL (ASCP) > Immunohistochemistry Supervisor > Strong Memorial Hospital > Department of Surgical Pathology > (585) 275-7210 > ________________________________________ > From: histonet-boun...@lists.utsouthwestern.edu > [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lin Bustamante > [lbustama...@cvm.tamu.edu] > Sent: Tuesday, October 05, 2010 4:58 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Osteoclast > > We need to find a way to stain mainly Osteoclast. > Any suggestions? > Thank you very much. > Lin > > Lin S. Bustamante, B.Sc.; HT(ASCP) > Research Associate > Histology Lab Supervisor > Veterinary Integrative Bioscience > Texas A&M University > College Station, TX 77843-4458 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet