We and several other sites (in British Columbia and Alberta) that I have contacted have been experiencing IHC staining problems. Ours started in August while others have been seeing this since May. We have had conference calls with Ventana about this and they have said that it is a problem with the SuperFrost Plus slides; the slides seem to be hydrophobic. The staining that we are seeing is negative controls/positive patient, positive control/negative patient as well as variable counterstain. We have control tissue on every slide. From what we have learned from Fisher and Ventana they are working together with Erie Scientific (manufacturer of the Plus slides) to find out what is causing the problem. The slides appear to be vary from box to box and within each box. Some lot numbers are worse than others; our most recent lot number was 062510-9. I would suggest that you contact your sale person as well as the platform vendor (if your staining is automated) so they are aware of how large the problem may be. We have not found another slide that is any better than the Plus. Ventana is working on an official communication for their clients. We are very frustrated with the lack of progress in solving the problem.
As anyone noticed that the Plus slides don't seem to have the same adhesive properties that they used to? Cathy Fitzpatrick Anatomic Pathology Section Head Kelowna General Hospital Kelowna, B.C. -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sris...@mail.holyname.org Sent: Thursday, October 07, 2010 7:32 AM To: histonet-boun...@lists.utsouthwestern.edu; Histonet@lists.utsouthwestern.edu Subject: [Histonet] problems with staining IHC Hi All, Our pathologists have made a complain that the staining of slides have been a problem. Same antibodies which stain one day does not work the following day. This has been going on for a few months. When we repeat stain they either work or not work and we have been sending them to reference labs. Our tech support person was here and told us that the slides ( fisher plus slides) have been a problem with some of her customers. Meanwhile our surrounding labs which are using the same slides have no issues. She showed us how the reagents were not spreading properly on the slides. Is anyone confronted this issue? If so, how was it solved? Thanks in advance. Mala Srishan Supervisor, Histology Holy Name Medical Center Teaneck NJ 07666 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Holy Name Medical Center is the recipient of: Magnet Recognition for Excellence in Patient Care, American Nurses Credentialing Center 100 Best Places to Work in Healthcare, Ranked Fourth Nationally by Modern Healthcare Best Places to Work in New Jersey, NJBIZ Awards for Emergency, Outpatient and Inpatient Service Excellence, J.D. Power Distinguished Hospital Awards for Clinical Excellence, HealthGrades Excellence Awards for Stroke, Gastrointestinal and Pulmonary Care, HealthGrades Best in Value Award, Data Advantage, LLC Chest Pain Center Accreditation, Society of Chest Pain Centers Primary Stroke Center Designation, The Joint Commission and NJ Department of Health and Human Services **** Warning: The information contained in this message is privileged and CONFIDENTIAL and is intended only for the use of the addressee above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution, or taking of any action in reliance on the content of this message is strictly prohibited. If you have received this communication in error, please notify the sender by replying to this message, and then delete it from your system. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet