Question: When doing frozen section on a lung mass (not fatty- just regular), and it chatters, what is the cause?
The cryostat was set at -26, we used the normal OCT media, and cut at 5microns..... I prefer not so cold (like -23 to -24) and I cut sections at 4, 5 , and even 6 to see if it would help... still chatter! The pathologist said it looked all necrotic-like!! Without knowing for sure if the tissue's morphology is due to its real state (ie- full of cancer) or a cause of freezing artifact, I need some help from any histotech that is a Frozen Section Guru :) Maria Katleba MS HT(ASCP) Pathology Dept. Mgr Queen of the Valley Medical Center 1000 Trancas Street Napa CA 94558 (707) 252-4411 x3689 direct (707) 226-4385 pager (707) 294-9229 cell- anytime Notice from St. Joseph Health System: Please note that the information contained in this message may be privileged and confidential and protected from disclosure. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
