We have developed a staining protocol for the CD79a antibody from two different vendors and both require a HIER TRIS/EDTA pH 9.0 retrieval.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amos Brooks Sent: Monday, November 01, 2010 2:34 PM To: jh...@vet.k-state.edu; histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC Staining Problem Hi, I think you mentioned using citrate retrieval. Have you tried high pH retrieval with EDTA or an equivalent? I think CD79 works better with high pH retrieval. Amos Message: 1 Date: Sun, 31 Oct 2010 19:29:59 +0000 From: Jennifer Hill <jh...@vet.k-state.edu> Subject: RE: [Histonet] IHC Staining Problem To: histonet <histonet@lists.utsouthwestern.edu> Message-ID: <8aa2173dc209ca438077a832ff98bd7f02806...@vetmxht.ads.vet.k-state.edu > Content-Type: text/plain; charset="iso-8859-1" Thanks to everyone who has responded so far. To answer the questions asked, our controls are either being cut the same day or the day or two before, so they are not setting for extended periods. We have also used at least three or four different controls, all with the same results. (The controls stain fine for other tests: CD20, CD3, etc.)The pathologists are saying that the patient slides are not staining as well. So it doesn't seem to be something so simple as a control issue. As to the dehydration/deparafinization, that takes place on an automated stainer, and no other stains are having issues so I don't believe that is the issue either. Jennifer Hill Kansas State University Veterinary Diagnostic Lab _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet