I would recommend methanol or ethanol for fixation, smears itself can be
culprit of inconsistent staining
Irena
> To: histonet@lists.utsouthwestern.edu
> From: heather.mcl...@webmail.co.za
> Date: Wed, 24 Nov 2010 19:42:51 +0200
> Subject: [Histonet] carnoys fluid
>
> Dear Histonetters,
>
> We do IHC on pap stained cyto smears. The results do not always "behave"
> accordingly on the smears that have been exposed to Carnoys. Does anybody know
> whether Carnoys Fluid affects IHC on cytology smears? I have seen papers that
> suggest improved IHC after Carnoys but maybe Histonetters feel difefrently?
> Thanks
> Heather
>
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