Peggy, Carson's modification of Millonig's buffer with formalin is great for kidney and any other general EM use for general pathology labs. I used it for many, many years when I worked in EM and it worked just as well as the specialized EM glutaraldehyde fixatives / buffers.
Just look up "Carson millonig" and you'll find several commercial sources. Or, of course, look at Carson's book for the formula. Tim Morken Supervisor, Histology, IPOX UCSF Medical Center San Francisco, CA, USA -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Lee & Peggy Wenk Sent: Saturday, December 18, 2010 4:26 AM To: Histonet Subject: [Histonet] EM cacodylate Need some help from Electron Microscopy people. What is the best substitute for cacodylate buffer (arsenic) when using on kidney and nerve biopsies? Least amount of protein clumping, little to now salt precipitate, good EM resolution? Thanks in advance. Peggy A. Wenk, HTL(ASCP)SLS William Beaumont Hospital Royal Oak, MI 48073 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet