We have always used water with liquid soap and some ammonia to soften blocks. It has had no effect on IHC.
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon.Davis-Devine Sent: Monday, December 27, 2010 3:58 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Use of ammonia water Happy Holidays Histonetters, I have a question for you. Do any of you use ammonia water to soften the blocks before cutting? If so, does this have any effect on IHC staining? Any help will be greatly appreciated. Thanks. Sharon Davis-Devine, CT (ASCP) Cytology-Histology Supervisor Carle Foundation Hospital Laboratory and Pathology Services 611 West Park Street Urbana, Illinois 61801 217-383-3572 sharon.davis-dev...@carle.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
