That depends on what staining you need to use. If an IHC, just fix the sections in acetone, air dry and proceed without HIER If H&E fix quickly with formalin 10%, wash → hematoxylin for 30 secs without differentiation → eosin 15 sec→ dehydrate and mount. Other HC procedures all will require fixation and using the specific protocol. Always remember that it is a frozen section and therefore more delicate and less adhered than a paraffin section. René J.
--- On Tue, 1/11/11, Blundon, Kimberly <kimberly.blun...@drdc-rddc.gc.ca> wrote: From: Blundon, Kimberly <kimberly.blun...@drdc-rddc.gc.ca> Subject: [Histonet] Cryostat help To: histonet@lists.utsouthwestern.edu Date: Tuesday, January 11, 2011, 10:28 AM Hello Histonetters, I am new to the histology world and I was hoping to get some feedback about staining after cutting sections in the cryostat. Does anyone have a protocol they use for staining after sectioning tissue in a cryostat? I have never done it before so I thought I would come to the experts. Thanks for your help! Kimberly _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet