Hello, A colleague is trying to determine the nucleic acid type of a rickettsial phage. Acridine orange with RNAse/DNAse treatment was not successful. They want to try a Schiff-methylene blue technique which requires hydrolyzing in Bouin's fluid (1 h at 60 C). Could anyone suggest another protocol which does not use picric acid (or suggest an alternative to Bouin's )?
Also, any advice for nucleic acid differential staining would be appreciated. Thanks very much, Carla Conway Histology Technician Western Fisheries Research Center, USGS 6505 N.E. 65th Street. Seattle, WA 98115-5016 USA Phone: 206-526-6282 ext. 242 Fax: 206-526-6654 E-mail: cmcon...@usgs.gov _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
