I've done many of these stains and I get my ORO stain from Poly Scientific. Actually I use the whole kit and I use Freida's protocol in the second edition. That includes fixing in 37% formaldehyde. I filter the ORO just before use and usually get nice clean slides. If there is a residue it isn't much and wipes off easily. I even re-use the ORO stain once in a while and it still comes out great. My slides are often used for quantifying the amount of lipids so I don't counterstain most of the time.
Andi Grantham On Jan 26, 2011, at 10:15 AM, Akemi Allison wrote: > Happy hump day! > > Does anyone have a good procedure for Oil Red O for FS on Muscle. > The procedure the lab I am working with is having a great deal of > problems with their muscle biopsy panel. I am trouble-shooting some > of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am > working with them with pH issues. > > The Oil Red O is very problematic. They are mixing before use and > filtering with 42 Watman paper, but there is a lot of residual > background on the slides. Also, they were not fixing the sections > with 37% formaldehyde, even though the procedure calls for it. Could > you share who you get the stain from also. Your assistance is > greatly appreciated. > > Akemi > > > Akemi Allison BS, HT (ASCP) HTL > Director > Phoenix Lab Consulting > Tele: 408.335.9994 > E-Mail: akemiat3...@yahoo.com > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet >
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