Hi Akemi, we don't do Oil Red O, but the Sudan III in an aceton-ethanol mixture. To prevent the precipitates on the slide, we stain in a plastic coplin jar with a screwed tap. The dye-precipitates accumulate on the ground. We take care not to mix them up while putting in the slide. So we need no filtering before staining. We only stain frozens of lung without fixation.
I dont know the formula of Oil Red O solution, but perhaps it can be handled like the sudan III. bye Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Akemi Allison Gesendet: Mittwoch, 26. Jänner 2011 18:16 An: histonet Betreff: [Histonet] Oil Red O for FS on Muscle Happy hump day! Does anyone have a good procedure for Oil Red O for FS on Muscle. The procedure the lab I am working with is having a great deal of problems with their muscle biopsy panel. I am trouble-shooting some of the issues for the 1-Step Trichrome 3.4 pH, NADH, AT Pase, I am working with them with pH issues. The Oil Red O is very problematic. They are mixing before use and filtering with 42 Watman paper, but there is a lot of residual background on the slides. Also, they were not fixing the sections with 37% formaldehyde, even though the procedure calls for it. Could you share who you get the stain from also. Your assistance is greatly appreciated. Akemi Akemi Allison BS, HT (ASCP) HTL Director Phoenix Lab Consulting Tele: 408.335.9994 E-Mail: akemiat3...@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet