Same with ETOH. We wipe our surfaces down with it to clean them so no cross contamination as we freeze our skin here on the metal side areas of our cryostats. If the alcohol hasn't been wiped dry or evaporated we have that problem too. Claire
________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Liz Chlipala Sent: Thu 2/10/2011 1:49 PM To: Reynolds,Donna M; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Help with OCT problem Donna I have seen soft or rather sticky OCT samples in the past. If you freeze in isopentane and don't let the isopentane evaporate off the samples prior to wrapping the sample in foil, that excess isopentane changes the OCT, it makes it sticky. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
