Before you freeze a muscle specimen, you coat it thinly with talc, pick it up with tweezers, and bob it up and down about ten times in the freezing liquid (not directly in liquid nitrogen), then mount it in OCT or other freeze-mount medium.
Correctly done, this will largely avoid bubble artifact. It takes a few times to get the knack of it - best to practice with some junk muscle (from a human amputation specimen, or from a dead animal of your choice) to get it right before you do it on an actual biopsy specimen or on valuable research material. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
