Thank you everyone for the helpful replies and info. I followed everyone's suggestions while waiting for service and didn't see any significant improvement. Once the service tech took everything apart he found the nozzle that controls dispensing of the bulk reagents was partially clogged causing the bulk reagents to be dispensed unevenly (usually totally off the slide!) Things straightened out afterwards. Thanks again everyone! Mike Dessoye
________________________________ From: Mark Tarango [mailto:marktara...@gmail.com] Sent: Wed 2/9/2011 11:58 AM To: Dessoye, Michael J Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Benchmark Problems Hi Mike, Did you wash the bottles and rinse them and then pump the water through the instrument before adding reagents and purging all again? We had a problem like this about 2 weeks ago. I think someone loaded the wrong bulk reagent onto the instrument. Cleaning it, purging with water and adding newly made bulk reagents fixed the problem for us. Mark On Wed, Feb 9, 2011 at 8:52 AM, Dessoye, Michael J <mjdess...@wvhcs.org> wrote: Hello, We started having a strange problem with our Ventana Benchmark XT. Out of the blue, our slides stopped staining. With most antibodies we get no reaction, some that were very strongly positive are now only very lightly staining. They do appear to counterstain OK. Following Ventana's recommendations we completely changed out all of our bulk fluids, purged the lines, changed antibodies and DAB kits. The last shot is some kind of instrument issue that's not triggering an error. Anyone ever see anything like this? Mike Dessoye _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you have received this email in error please notify the originator of the message. This footer also confirms that this email message has been scanned for the presence of computer viruses. Any views expressed in this message are those of the individual sender, except where the sender specifies and with authority, states them to be the views of Wyoming Valley Health Care System. Scanning of this message and addition of this footer is performed by Websense Email Security software in conjunction with virus detection software. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
