Jo-Ann, Nothing is changed, but storage in PBS does not affect neither morphology nor antigens. I used to keep some neutral formalin- or formaldehyde-PBS fixed specimens up to 1 year in sterile PBS at +4 C without any visible changes. So - don't worry. It will be fine.
Anatoli Gleiberman, PhD Director of Histopathology Cleveland Biolabs, Inc 73 High Street Buffalo, NY 14203 phone:716-849-6810 ext.354 fax:716-849-6817 e-mail: agleiber...@cbiolabs.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jo-Ann Bader, Ms. Sent: Wednesday, February 23, 2011 3:57 PM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet] Short term tissue storage PBS??? Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens are stored, short term in 70% alcohol, until you are ready to process them. Has something changed lately that I am not aware of? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 (3355) Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This communication may contain privileged information. It is intended solely for the use of the addressee. If you are not the intended recipient, you are strictly prohibited from disclosing, copying, distributing or using any of this information. If you received this communication in error, please contact the sender immediately and destroy the material in its entirety, whether electronic or hard copy. This communication may contain nonpublic information about individuals and businesses subject to the restrictions of the Gramm-Leach-Bliley Act. You may not directly or indirectly reuse or redisclose such information for any purpose other than to provide the services for which you are receiving the information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
