We trim to 2mm thick. Jan Shivers UMN Vet Diag Lab
----- Original Message ----- From: "Liz Chlipala" <l...@premierlab.com> To: "Sherwood, Margaret " <msherw...@partners.org>; <histonet@lists.utsouthwestern.edu>
Sent: Thursday, March 17, 2011 3:02 PM Subject: RE: [Histonet] Processing animal fat 5mm is pretty thick still. We process skin and fat and we use a longer processing cycle. 1 to 1.5 hours per station. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sherwood, Margaret Sent: Thursday, March 17, 2011 1:57 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Processing animal fat My question is directed specifically to veterinary histologists or histologists who also do a fair amount of animal processing. We are having a terrible time processing pig fat. We had problems previously, but thought we had solved them. This latest project (pig skin with a lot of fat attached) came out awful. The fat was not adequately processed: couldn't section it, it just crumbled. In the block, it appears white and crumbly. The funny thing is some blocks came out all right, but most didn't. PLEASE help! Let me know how you process your animal fat (sp. Pig)! Is there a size issue (we trim it if it is greater than 5mm)? We have gotten help from the histonet before and instituted these suggestions (i.e. let sit in formalin for 48 hours; put in cassettes in a 60 degree oven for 1-2 hours and then cool to room temp. Then process using a 1 hour program). Any help would be appreciated. Thanks! Peggy Peggy Sherwood Lab Associate, Photopathology Wellman Center for Photomedicine (EDR 214) Massachusetts General Hospital 55 Fruit Street Boston, MA 02114-2696 617-724-4839 (voice mail) 617-726-6983 (lab) 617-726-1206 (fax) msherw...@partners.org The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Partners Compliance HelpLine at http://www.partners.org/complianceline . If the e-mail was sent to you in error but does not contain patient information, please contact the sender and properly dispose of the e-mail. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet