Vendor sheets may not be accurate we keep an internal log of all of our antibodies and what species we have tested them in. So far in our lab we have found several antibodies that the vendor states works in a species and it does not in our hands, in these cases we always contact the vendor, basically for a refund and they have updated their specification sheets to reflect the change. We do use protein atlas a lot for verification of the IHC protocols and tissue staining patterns.
Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC PO Box 18592 Boulder, Colorado 80308 office (303) 682-3949 fax (303) 682-9060 www.premierlab.com Ship to Address: 1567 Skyway Drive, Unit E Longmont, Colorado 80504 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of shive...@umn.edu Sent: Friday, March 18, 2011 1:06 PM To: Truscott, Tom Cc: Histonet; Morken,Timothy Subject: Re: [Histonet] RE: comprehensive list? I've also found that occasionally the antigen retrieval method quoted on a vendor's data sheet doesn't unmask antigens as well as another method tried in my initial antibody validation trials with that antibody. So, my point is... any comprehensive list should be used only as a guideline; results in your lab will be affected by the reagents, equipment, and environmental conditions you employ in your own lab. Jan Shivers UMN Vet Diag Lab On Mar 18 2011, Truscott, Tom wrote: Yes, vendor sheets are good, but not all give enough info, and you may have to compare several vendor, and as you say, antibodies only tested by western blot are common in research. Yet, it would be a great money and timesaver if there was one site to go to, to see if anyone had tried these antibodies by different methods and could also submit their own results for entry, possibly with links to Journal articles, much like what some vendors do. It could be a quick reference guide to setting up some research or double staining methods, or just see if some new request is going to fit your routine. Someone would have to have more time and computer skills than I. Thanks all for the input, TOM T > >-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Morken, Timothy >Sent: Friday, March 18, 2011 10:38 AM >To: Histonet >Subject: [Histonet] RE: comprehensive list? > >Tom, > Vendor datasheets are the best source of information. The vast majority of clinical vendors antibodies are designed for formalin fixed paraffin embedded tissue. Research vendors are all over the map and often only test by western blots or elisa so they may or may not have information about how their antibodies work in fixed tissue. > >Tim Morken >Supervisor, Histology, IPOX >UCSF Medical Center >San Francisco, CA, USA > > >-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom >Sent: Friday, March 18, 2011 9:02 AM >To: Histonet >Subject: [Histonet] comprehensive list? > Hi All, Is there a comprehensive list in one location somewhere that gives information on all or most antibodies on how they work with FFPE or FS or other fixatives, so that we don't have to try to many approaches when we get a new antibody to try. Thanks, Tom T >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > >_______________________________________________ >Histonet mailing list >Histonet@lists.utsouthwestern.edu >http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet