You might try Background Sniper from Biocare or Background Buster from Innovex - we process animal tissues exclusively and we use several rabbit primary antibodies.
Tresa Goins, Ph.D. Histopathology Supervisor Department of Livestock Bozeman, Montana -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of sgoe...@mirnarx.com Sent: Tuesday, March 22, 2011 3:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Stupid Rabbit primaries! So I haven't had to deal with rabbit polyclonal primaries in a long time because I remember how much the background sucks with them. Unfortunately the only available antibody is a rabbit polyclonal. Does anyone have any suggestions for how to eliminate the background? I have diluted almost to the point of the antigens not showing! Thanks guys and gals!! Sarah Goebel, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
